天津地区鸡致病性大肠杆菌血清型分布及其优势血清型的外膜蛋白型研究

Studies on Distribution of Serotypes of Chicken Pathogenic Escherichia coli and Outer Membrane Protein Patterns of Predominant Serogroups in Tianjin Regions

从天津地区各区县养鸡场分离 ,鉴定出 45株鸡致病性大肠杆菌。对 45株致病性大肠杆菌进行血清型鉴定 ,共定型出 3 1株 ,分属 1 4个血清型 ,其中 O78、O88、O2 、O4 5、O53、O14 5为优势血清型 ,占定型菌株的 58.3 %。提取 O78、O88、O2 、O4 5、O53和O14 56个血清型 1 8个分离株的外膜蛋白 ( Outermembrane protein,OMP) ,测定外膜蛋白型 ( Outmembrane protein patterns,OMPS) ,SDS-PAGE分析表明这些分离株共产生了 3个 OMP型 ( 1～ 3型 )。 OMP-1型为 O78、O88、O2 、O53、O14 55个血清型分离株所共有 ,OMP-2型为 O78、O4 5、O14 53个血清型分离株所共有。这表明同一血清的菌株可能属于完全不同的 OMP型 ,而血清型不同的菌株也可能为同一 OMP型。

45 strains of Pathogenic Escherichia coli(E.coli) were isolated from chickens of fowl farms in Tianjin regions and were studied for O serotypes. 31 of them were identified as O type belonged to 14 serogroups and the O78,O88,O2,O45,O53and O145were Predominant.Outer membrane proteins (OMP) were prepared from 18strains belonged to O78,O88,O2,O45,O53 and O145 serogroups. SDSPAGE analysis showed that 3 OMP patterns (1~3 patterns) were found from these strains. OMP1 pattern was found from the O78,O88,O2,O53 and O145 serogroups,OMP2 pattern was found from the O78,O88 and O2 serogroups, OMP3 pattern was isolated from O78,O45, and O145 serogroups. These results showed that strains of different O serogroups of chicken E.coli could have the same OMP pattern and the strains belonged to the same O serogroup could have different OMP patterns.