应用白细胞介素-1β和肿瘤坏死因子α抗体预防术后腹腔粘连实验研究

Prevention of Postoperative Abdominal Adhesions by IL-1β and TNFα Antibody in Mice

目的 研究白细胞介素 -1β(IL -1β)和肿瘤坏死因子α(TNFα)抗体预防小鼠术后腹腔粘连的能力。 方法 以BALB/c小鼠为动物模型 ,60只BALB/c小鼠随机分成四组 :生理盐水对照组 ,IL -1β抗体治疗组 ,TNFα抗体治疗组 ,IL -1β联合TNFα抗体治疗组 ,经过一同一标准的腹膜损伤手术后 ,分别在关腹前腹腔内灌注生理盐水及相应抗体。术后 2 0d将小鼠处死 ,观察腹腔内粘连的情况 ,通过对粘连的程度、位置以及粘连类型的综合评价得出粘连分数。结果 IL -1β联合TNFα抗体治疗组的平均粘连分数明显低于对照组 (P <0 0 1) ,2～ 3级粘连发生率比对照组亦有明显减低 (P <0 0 1) ,无一例小鼠出现切口疝。结论 IL

Objective To assess the ability of interleukin-1 beta (IL-1β) antibody and tumor necrosis factor alpha (TNFα) antibody to prevent postoperative abdominal adhesion formation in mice.Methods 60 BALB/c mice were randomly divided into four groups: normal saline group(n=15), anti-IL-1β group(n=15), anti-TNFα group(n=15),and anti-IL-1β combined with anti-TNFα group(n=15). After an identical peritoneal injury operation, mice were treated using intraperitoneally with either normal saline or corresponding antibody at the time of abdominal closure. The mice were killed 20 days after operation, the abdominal incisions and the development of intra-abdominal adhesions were observed. Adhesion scoring was based on an overall assessment of the extent,location, and type of adhesion.Results Anti-IL-1β combined with anti-TNFα group had significantly lower adhesion scores (0 84±0 19) than that in other groups(P<0 01). No incisional hernia was found in abdominal wall in all cases.Conclusion The intraperitoneal administration of IL-1β and TNFα antibody could inhibit postoperative adhesion formation.