骨骼肌卫星细胞移植治疗心功能衰竭的实验研究

Investigation of Cardiac Failure Therapy by Transplanting Skeletal Satellite Cells

目的 移植骨骼肌卫星细胞 (SC)修复受损心肌 ,治疗心功能衰竭。方法 雄性苏中幼猪 2 3头 ,随机分为实验组与对照组。结扎对角支制作急性心肌梗死模型 ,造成心衰。实验组采用改良Dorfman法培养SC ,心肌内注射种植 ;对照组注射等量细胞培养液。通过病理形态观察探讨SC移植对心衰的治疗作用。结果 ⑴存活 10头猪 ,实验组 5头 ,对照组 5头。总制模成功率43 5 % ;⑵SC贴壁生长 ,延迟分瓶或降低培养液中胎牛血清 (FBS)浓度 ,SC生长停滞 ,相互融合形成肌管 ;⑶实验组梗死区内见新生的横纹肌纤维 ,细胞间无闰盘 ,毛细血管密度明显高于对照组。结论 自体SC移植后可在心肌内存活 ,可能通过改变心肌顺应性、心脏构型 。

Objective Satellite cells(SCs) transplantation was tried to improve cardiac function in this experiment.Methods A myocaridium infarction was created in 23 male Suzhong pigs by ligating the diagonal ramus,and the alive pigs were divided into the experimental group and the control group.SCs were extracted,purified,cultured by modified Dorfman method,and were transplanted into myocardium by intramyocardial injection.By pathomorphological research,we observed the therapeutic outcomes of heart failure.Results ⑴10 pigs were alive(5 in the control group,and the other 5 in the experimental group).The successful rate of making mould was 43 5%.⑵After cultured in vitro for 3 days,SCs were spindle-shaped,and sticked to walls.SCs growth were stasis and each other fusing into form myotube when we delayed to divede Petri dishs or reduced the concentration of Fetus Bovine Serum(FBS) in the culture medium.⑶In the experimental group,neonate skeletal muscle cells were observed in the infarction regions,which were multi-nuclei near edges.These cells aligned in an accordance direction.Intercalated disks were not found between them.The capillary density in experimental group was much higher than the control group.Conclusions Autograft of SCS can be alive in acceptor,and improve the heart function by altering cardiac compliance and configuration,or by stimulating the blood capillary proliferation.SCs don't form intercalated disks with remnant myocardial cells,so synchro-contraction will not occur between them,but malign arrhythmia are not found.