阿霉素冲击诱导人骨肉瘤多药耐药细胞模型

Experiment Models for the Study of MDR:Human Osteosarcoma Cell Sublines

目的 :建立人骨肉瘤细胞多药耐药亚系。方法 :采用ADM间断冲击法诱导骨肉瘤细胞 ,并用免疫荧光、MTT及ABA法检测肿瘤细胞的耐药特征。结果 :本实验建立了 6株多药耐药细胞亚系MG 6 3/R1～ 6。用免疫荧光术可以检测到P gp的表达 ,MTT、ABA法显示各亚系细胞的多药耐药性明显增加 ,并且维拉帕米可以拮抗P gp的作用。结论 :MDR1/P170 在多药耐药的特性上起着至关重要的作用 ,而且这些骨肉瘤多药耐药细胞亚系为进一步研究骨肉瘤耐药特征及逆转方法打下基础。

Objective:To establish a multidrug-resistene(MDR) subline of human osteosarcoma.Methods:The authors created cell lines by short-time pulse exposure of the parent cell line to adriamycin followed by single-cell clone.MDR character was detected with immunoflorescence method and MTT,ABA Results:The authors developed six P-gp-positive,human osteosarcoma cell lines that were resistant to adriamycin MG-63/R 1～6which were higher than the cells from parent line respectively and also cross-resistant to other drugs at different degrees.There was less accumulation of the drug in resistent cell lines,AB% of MG-63/R 6 is only 9.8%.but when verapamil is used,AB% could risen to 52%.Immunoflorescence can detect significant high expression of P-gp in MDR cell lines.Conclusion:Expression of MDR1/P 170is key factor to regulate MDR phenotype of osteosarcoma.These newly described multidrug-resistent osteosarcoma cell sublines are useful models for further characterization of drug resistence in osteosarcoma and for the development of treatment protocols.