油菜诱变和离体草酸筛选抗菌核病材料

In vitro screening stem rot resistant (tolerant) materials in Brassica napus L

以油菜华双 3号为材料 ,研究辐射诱变和离体草酸处理筛选抗菌核病材料的方法和技术。结果表明 ,用于离体筛选种子的辐照剂量以 40～ 12 0Krad为宜 ;用草酸处理60 Co -γ射线照射种子产生的试管苗 (一轮草酸筛选 ) ,或用草酸处理辐照种子及种子产生的试管苗 (两轮草酸筛选 ) ,获得 49株抗草酸小苗 ;未经辐射处理的种子均未筛选出抗草酸小苗。将抗草酸小苗离体叶接种核盘菌菌丝体 ,获得 9株抗 (耐 )核盘菌的小苗。

The experiment attempt to create stem rot-resistant rape materials through the way of inducing mutation and in vitro screening with the oxalic acid toxin. Dry seeds of B.napuscultivar Huashuang No.3 were exposed to gamma rays at doses of 40～480Krad. The irradiated seeds were cultured on B 5 medium added the selection agent oxalic acid of 1.0～ 5.0mmol/L to conduct the first cycle screening. Survived plantlets (2 leaves stage) were transplanted into the B 5 medium with 1.0～4.0mmol/L oxalic acid to treat for 4 days. The resistance (tolerance) of plantlets to stem rot were identified by inoculating the mycelia of S.sclerotiorumon leaves of plantlets screened. The results indicated that the doses of 40～120 Krad γ-rays can be used to irradiate rape seeds for resisting oxalic acid. The oxalic acid is a suitable selective agent for resistance screening in vitro; stem rot resistant or tolerant 9 plants were selected from 49 tube seedlings with resistance to oxalic acid.