芦荟芽苗快繁及试管苗栽培驯化研究

A Study on the Rapid Regeneration and Plantlet Cultivation Under in Vitro Culture of Aloe (Aloe vera L. var, Chinensis (haw) Berger)

以中华斑纹芦荟(AloeveraL.var.chinensis(Haw.)Berger)为试验材料,研究了芦荟的组织培养、快速繁殖和试管苗驯化及栽培技术。结果表明,短缩茎诱导愈伤组织效果较好。芽苗快速繁殖阶段以MS+6-BA2.0+NAA1.0+Su50g/L为最佳;试管苗栽培驯化中基质以珍珠岩∶石至石∶砂子为1∶1∶2最佳;活性炭对防止根变态有重要作用。

Using aloe (Aloe vera L. var, Chinensis (haw) Berger), the rapid regeneration and plantlet cultivation were studied. The result showed that the shortened stem had a better effect for callus initiation. Medium MS +6BA2.0 + NAA1.0+Su 50 g/L was suitable for shoot multiplicatio. Mergarite∶ vermiculite∶ sand (1∶1∶2) fits for plantlet cultivation. Active carbon can prevent root becoming abnormal.