陕西省咸阳市、商州市烟草病毒病毒原鉴定初报

Preliminary Identification of Tobacco Viruses in Xianyang and Shangzhou City of Shaanxi Province

2001年在咸阳市、商州市采集了101个毒株,经过生物学测定、血清学反应(琼脂双扩散法和酶联免疫吸附法)、电镜观察鉴定出5种为害烟草的病毒,它们是烟草普通花叶病毒(TMV)、黄瓜花叶病毒(CMV)、烟草蚀纹病毒(TEV)、马铃薯Y病毒(PVY)、烟草环斑病毒(TRSV)。其中烟草普通花叶病毒病、黄瓜花叶病毒病和烟草蚀纹病毒病是当前生产上发病率较高、造成损失较大的3种病害;同时,烟草普通花叶病毒(TMV)和黄瓜花叶病毒(CMV)、烟草普通花叶病毒(TMV)和马铃薯Y病毒(PVY)的复合侵染也是当前生产上亟待解决的主要问题。

101 virus samples were collected in tobacco producing regions in Xianyang City and Shangzhou City of Shaanxi province in 2001. Five viruses were identified by biological determination, serological assay (Double diffusion and Enzymelinked immiunosorbbent assay) and microscopy observation. They were tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), tobacco etch rirus (TEV), patato virus Y (PVY) and tobacco ringspot virus (TRSV). Tobacco mosaic virus (TMV), cucumber mosaic virus (CMV) and tobacco etch rirus (TEV) were the most common viruses and caused serious losses in fields. Simultaneously the complex infection of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV), patato virus Y (PVY) and tobacco mosaic virus(TMV) are also a crucial problem to be resolved at present.