新几内亚凤仙组培快繁技术研究

Study on Fast Propagations of New Guinea Impatiens with Tissue Culture Method

通过对新几内亚凤仙组织培养及入土移栽的技术研究,分别筛选出诱导芽分化的最适培养基和诱导生根的最适培养基。试验结果表明,不同的激素浓度对新几内亚凤仙组培苗的生长效果有着较大的影响,最适宜的诱导芽分化的培养基为MS +NAA 0.3mg·L-1+6 -BA 0.8mg·L-1,诱导率为 90 %;最适宜的生根培养基为 1/2MS +NAA 0.3mg·L-1,生根率为 93.3%;最适宜的移栽基质为蛭石 +粗砂(1∶1),成活率为 87%。

Through the techniques of fast propagation and explanting New Guinea Impatiens into soil, the optimal culture medium inducing bud and root and the optimal transplanting substratum were selected. The results show that the different density of hormone could greatly affect the growth of New Guinea Impatiens, the optimal medium inducing bud was MS+NAA 0.3 mg·L -1 +6-BA 0.8 mg·L -1 and the inducing rate was above 90%.The optimal medium inducing root was 1/2 MS+NAA 0.3 mg·L -1 , and the inducing rate was above 93.3%. The optimal transplanting substratum was vermiculite + coarse sand (1∶1) and the survival rate was above 87%.