摘要
以蝴蝶兰无菌幼苗为外植体,在分株增殖培养基(1/2MS + 6-BA 3.0mg/L + NAA 0.2mg/L + CM20g/L)上培养30d后,形成幼小丛生植株;将此幼小植株移至生长培养基(1/2MS + 6-BA 1.0mg/L + NAA 0.1mg/L)上,30d后便形成具3～4片叶与数条粗壮根的较大植株,移栽成活率达80%以上。
The test tube seedlings of Phalaenopsis were cultured on division propagation media supplemented with 1/2MS + 6-BA 3.0mg/L + NAA 0.2mg/L + CM 20g/L. After 30 days, the thick plantlets produced. The plantlets grew into plants with 3～4 leaves and several strong roots in growth media supplemented with 1/2MS + 6-BA 1.0mg/L + NAA 0.1mg/L in 30 days. The transplant survival percent was more than 80%.
出处
《亚热带植物科学》
2003年第1期23-25,共3页
Subtropical Plant Science
基金
福建省教育厅资助项目(JB01099)
关键词
蝴蝶兰
组织培养
分株繁殖
Phalaenopsis
tissue culture
division propagation.
