菊花转抗虫基因植株的PCR快速鉴定

Rapid PCR identifying of transgenic plants of chrysanthemum integration Bt gene and GNA gene

对经过卡那霉素抗性筛选所得到的菊花转苏云金芽胞杆菌毒蛋白Bt基因和转雪花莲外源凝集素GNA基因所得的抗性植株 ,利用改良SDS法提取菊花DNA ,然后利用NPTII引物对 6个菊花品种的抗性植株所提DNA进行PCR扩增 ,发现了符合NPTII基因片段大小的亮带 ,初步证实获得了转Bt基因和转GNA基因的菊花植株。

Resistant plants of chrysanthemum that transfered Bt gene and GNA gene were gained successively on selective media containing kanamycin,chrysanthemum DNA was isolated to use method of improving SDS PCR were proceeded with NPT II primers,and PCR products produced identical banding patterns representing the coding region of NPT II gene Transgenic plants of chrysanthemum integrating Bt gene and GNA gene were primaryly identified