表面质膜共振技术——一种检测蛋白质特异结合反应的方法

Surface plasmon resonance (SPR) technique-A method to detect proteins' specific binding interactions

表面质膜共振 (SPR)技术是一种监测生物大分子之间特异结合反应的物理方法。该文综述了SPR技术的特点 ,操作方法 ,光学结构及监测原理 ,数据采集及分析方法 ,应用范围其存在的问题等。同时 ,展望了该技术在昆虫学研究中广阔的应用前景。

Surface plasmon resonance technique is a physical method to measure the special binding affinities of biological macromolecules. This article reviews SPR system characterization, experimental methods, optical configuration and detection principles, data collection and analysis models, fields of application and the problem of quantitative determination in the application of this technology. The broad prospects for the application of SPR techniques in entomological research are also discussed.