阿司匹林对脂质过氧化损伤红细胞变形能力和膜收缩蛋白变化的影响

EFFECT OF ASPIRIN ON ERYTHROCYTE DEFORMABILITY AND SPECTRIN WITH LIPID PEROXIDATIVE INJURY

①目的 探讨阿司匹林 (AS)对脂质过氧化损伤红细胞变形能力和膜收缩蛋白的影响。②方法 以黄嘌呤氧化酶 (HXO)氧自由基体系和活性氧 (H2 O2 )作用于细胞 ,观察AS对红细胞溶血度、红细胞膜脂质过氧化物 (LPO)、红细胞变形能力 (ED)、红细胞收缩蛋白二聚体 (SP D)和四聚体 (SP T)的影响。③结果 AS能明显降低红细胞溶血度 ,与对照组比较差异有显著性 (t=2 .3 91～ 8.5 91,P <0 .0 1、0 .0 0 1)。在HXO体系作用下 ,红细胞膜LPO、红细胞滤过指数 (EFI)、SP D和SP D/SP T明显增高 ,SP T明显降低 ,与对照组比较差异有极显著性 (t=17.65 3～ 2 6.60 9,P <0 .0 0 1)。在不同浓度AS作用下 ,红细胞膜LPO、EFI、SP D和SP D/SP T明显降低 ,SP T明显增高 ,与对照组比较差异均有显著性 (t=3 .0 61～ 16.5 5 9,P <0 .0 1、0 .0 0 1)。④结论 AS在红细胞水平上能直接对抗氧自由基引发的脂质过氧化损伤 。

Objective To explore the effect of aspirin (AS) on erythrocyte deformability (ED) and spectrin with lipid peroxidative injury. Methods Erythrocytes were treated by hypoxanthine xanthaine oxidase (HXO) free-radical system and active oxygen (H 2O 2), the effect of AS on hemolysis, erythrocyte membrane lipid peroxide(LPO), ED, erythrocyte membrane spectrin dimers (SP-D)and tetramer(SP-T)were observed. Results AS could significantly decrease the erythrocyte hemolysisas compared with the control group (t=2.391-8.591,P<0.01,0.001). Under the effects of HXO system, the erythrocyte membrane LPO, erythrocyte filtration index (EFI), SP-D, and SP-D/SP-T were significantly higher, SP-T was significantly lower than those for the control (t=17.653-26.609,P<0.001), the erythrocyte membrane LPO, EFI, SP-D, and SP-D/SP-T were lower and SP-T was higher than those for the control (t=3.061-16.559,P<0.01,0.001). Conclusion AS could directly counteract the erythrocyte membrane lipid peroxidative injury caused by free radicals, protect SP and improve ED.