SLE病人外周血淋巴细胞早期凋亡状态的检测

A DETECTION OF EARLY APOPTOSIS OF LYMPHOCYTES IN THE PERIPHERAL BLOOD OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

①目的 探讨淋巴细胞凋亡在系统性红斑狼疮 (SLE)发病机制中的作用。②方法 应用AnnexinⅤ试剂盒及流式细胞仪 (FCM)对SLE病人及正常人外周血淋巴细胞 (PBL)进行早期凋亡状态的检测 ,并采用免疫比浊法检测SLE病人补体C3含量。③结果 SLE活动期病人PBL早期凋亡率明显高于稳定期病人及正常人 (F =3 6.68,q =3 .17、3 .63 ,P <0 .0 1) ;稳定期病人与正常人相比无显著性差异 ( q =0 .15 ,P >0 .0 5 ) ;活动期病人中初发病组与已用糖皮质激素或免疫抑制剂治疗组相比无差异 (t=0 .2 1,P >0 .0 5 )。活动期病人PBL凋亡率与补体C3含量呈高度负相关 (r =- 0 .85 9,P <0 .0 1)。④结论 SLE病人外周血大量凋亡淋巴细胞可能通过释放过量自身抗原的方式诱导一系列自身抗体的产生 。

Objective To study the role of apoptosis of lymphocytes in the pathogenesis of systemic lupus erythematosus (SLE). Methods The levels of early apoptosis of lymphocytes of peripheral blood(PLB) in SLE patients and healthy people were assessed by Annexin Ⅴ and flow cytometry(FCM). The complement (C3) of SLE patients was detected quantitatively by turbidimetry. Results The percentage of the early apoptotic lymphocytes in the peripheral blood of active SLE patients was significantly higher than that of the inactive SLE patients and of the control group (F=36.68, q=3.17, 3.63,P<0.01), but there was no marked difference between quiescent SLE and normal healthy people (q=0.15, P>0.05). Of the active SLE patients, no difference was found between the incipient and the treated with corticosteroid or immunosuppressive therapy (t=0.21, P>0.05). The rate of apoptosis negatively correlated with the complement in active SLE patients (r=-0.859, P<0.01). Conclusion The early apoptotic lymphocytes increase significantly in the peripheral blood of SLE patients, which may contribute to the pathogenesis of SLE by producing excessive amounts of autoantibodies.