胶原在圆锥角膜中的表达

EXPRESSION OF COLLAGEN IN KERATOCONUS

①目的 观察正常角膜及圆锥角膜组织中Ⅰ～Ⅵ型胶原的表达 ,探讨各型胶原在圆锥角膜中的变化。②方法 采用免疫组织化学SABC法检测 7例正常角膜和 2 1例圆锥角膜组织中Ⅰ～Ⅵ型胶原的表达。③结果 Ⅰ和Ⅲ型胶原在正常角膜和圆锥角膜的上皮层、实质层及内皮层均有阳性表达 ,但圆锥角膜比正常角膜表达得弱 ;而圆锥角膜基质斑块状瘢痕区可见胶原Ⅲ型呈强阳性表达。Ⅱ型和Ⅴ型胶原在Bowman层及基质层表达 ,正常及圆锥角膜中无明显差异 ,但在圆锥角膜瘢痕区Ⅴ型胶原表达呈强阳性。Ⅵ型胶原的表达在Bowman层、De scemet层和基质层均可检测到 ,但圆锥角膜中Ⅵ型胶原表达减低 ,特别在圆锥角膜的瘢痕区内 ,Ⅵ型胶原表达明显降低。圆锥角膜组织中Ⅳ型胶原的阳性表达在Bowman层及Descemet层 ,依角膜病变的程度而呈不同程度的减弱 ;Bowman层的断裂呈波浪状 ,Descemet膜破裂并内卷。④结论 Ⅰ～Ⅵ型胶原的表达在圆锥角膜的发病过程中有明显的变化 ,胶原成分的减少会导致角膜的机械抵抗力减弱 ,并使角膜前凸变薄 ,最终影响角膜的正常形态 。

Objective To observe the expression of collagen type Ⅰ-Ⅵ in normal cornea and keratoconus and study the changes of various types of the collagen in keratoconus.\ Methods\ Immunohistochemical SABC method was used to detect the expression of collagen type Ⅰ-Ⅵ in 7 normal corneae and 21 keratoconus. \ Results\ Collagen type Ⅰ and Ⅲ were expressed in epithelium,endothelium and stroma in normal cornea and keratoconus. The expression showed weakly in keratoconus. Collagen types Ⅱ and Ⅴ were expressed in Bowmans layer and stroma in normal cornea and keratoconus. The positive expression decreased in keratoconus, too. But there was strong expression in scar area of keratoconus. Collagen type Ⅵ was expressed in Bowmans layer, Descemets layer and lamellar layer. But the expression decreased in keratocinus, especially in scar area. Collagen type Ⅳ was expressed in Bowmans layer and Descemets membrane in normal cornea and keratoconus. The positive expression decreased with various corneal lesions. Bowmans membrane was wavily and intermittently broken and Descemets membrane was broken as well. Conclusion\ Abnormal expression of type Ⅰ-Ⅵ can be found in the onset of illness. The decrease of collagen may weaken the stability of stroma lamellar and cornea shape to conus. The onset of keratoconus is closely related to collagen.