人类肿瘤细胞诱导分化模型的建立

THE MODEL OF HUMAN TUMOR-CELL-INDUCING DIFFERENTIATION

①目的 建立肿瘤细胞诱导分化的模型。②方法 应用 1× 10 -6、1× 10 -5mol/L全反式维甲酸(ATRA)和体积分数 0 .0 0 5、0 .0 10二甲基亚砜 (DMSO)分别与人急性早幼粒白血病细胞株 (HL 6 0 ) (2× 10 8～5× 10 8/L)共同培养 5d ,对照组加入与诱导剂等体积的无水乙醇 ,监测HL 6 0细胞形态学、四氮唑蓝 (NBT)还原反应、细胞周期动力学变化。③结果 HL 6 0细胞经ATRA作用后出现分化现象 ,第 3天NBT阳性细胞增加 ,第 4天达高峰 ,第 3～ 5天无明显差异。 1× 10 -6与 1× 10 -5mol/LATRA诱导分化作用无明显差别。以体积分数 0 .0 10的DMSO诱导HL 6 0细胞分化作用最好 ,而且随时间的延长其效应逐渐增强 ,第 5天达高峰。细胞周期分析显示 ,HL 6 0细胞经ATRA诱导分化后 ,G0 +G1期细胞增加 ,S期细胞减少 ,G2 期细胞稍有减少。④结论 采用ATRA和DMSO诱导HL 6

Objective To set up a cell model of human tumor cell inducing differentiation. \ Methods\ The HL 60 cells were cultured with 1× 10 -6 and 1×10 -5 mol/L all trans retinoic acid (ATRA) and 0.005, 0.010 dimethyl sulfoxide (DMSO), respectively, for 5 days. The controls were cultured with the same volume of ethanol. NBT reduction and cytometry analysis were used to examine the differentiation of HL 60 cells.\ Results\ Both ATRA and DMSO inhibited the growth of HL 60 cells, arrested cells in G 0+G 1 phase, and induced cells to differentiation. The effects of NBT reduction increased. The differentiation features of ATRA treated HL 60 could be observed on the first day and there was little difference in the third, the fourth and the fifth day. The dosage of 1×10 -6 and 1×10 -5 mol/L had almost the same effects. The effects of DMSO were the strongest on the fifth day and the dosage of 0.010 DMSO was the most effective among 0.005, 0.010 ones. \ Conclusion\ Both ATRA and DMSO are effective inducers to HL 60 cell differentiation.