北京油葫芦卵子发生中DNA及RNA动态变化研究

Variation of DNA and RNA contents in the oogenesis of Teleogryllus mitratus

用孚尔根及甲基绿 -派洛宁组织化学染色法了解北京油葫芦Teleogryllusmitratus(Burmeister)卵子发生各时期阶段中卵内DNA及RNA动态变化规律。在卵子发生的最初阶段 ,核中DNA的合成和复制最活跃 ,以后便慢慢减弱 ;而RNA则在第 2阶段合成最旺盛。在卵子发生各个阶段 ,滤泡细胞中DNA ,RNA均为阳性反应 ,并在卵细胞的卵黄形成期活动旺盛 ,为卵母细胞卵黄蛋白形成提供物质基础。卵子发生第 4～ 6阶段 ,滤泡细胞开放时期 ,血淋巴内一些物质可能直接或间接通过滤泡细胞间隙进入卵母细胞内 ,参与卵母细胞的发育和构建。研究表明卵子发生初期卵母细胞的发育和物质构建主要以内源性合成积累为主 ,中后期则有外源性物质的参与。

Active variation of DNA and RNA in the oogenesis of Telegryllus mitratus (Burmeister) was investigated using histochemical methods. The results indicate that DNA synthesis and transcription are mostly active in the oocyte nucleus during the first stage of oogenesis. Transcription then slows down with oocyte development.The RNA of the ooplasm is active mostly in translation during the third stage of the oogenesis. Compared with the former, the DNA and RNA of follicular cells always maintained vigorous transcription and translation activity during most stages of oogenesis, especially during the proteineous yolk formation from stages four to six. This phenomenon shows that follicular cells do not only satisfy their own requirements in division and reproduction but also provide some useful information and materials for oogenesis. In stages four to six of oogenesis, some information and substances deposited in blood lymph may take part oocyte construction directly or indirectly through the follicular cell. The results also indicate that, during the primary stage, the oocyte mainly depend on endogenous information and substances. But during middle and later stages, some exogenous substances also participate the oocyte development.