摘要
A novel tapetum-specific cDNA clone of rice, its corresponding gene designed as RA39, is isolated by RNA subtractive hybridization, differential screening and rapid amplification of cDNA ends. The RA39 cDNA is 1013 bp in length with an open reading frame encoding 298 amino acid residues. mRNA in situ hybridization reveals that RA39 is a tapetum-specific gene, and highly expressed at the meiosis stage of pollen mother cells. The deduced protein contains a signal peptide, a transmembrane region and a cytoplasmic tail, and is predicted to localize in endoplasmic reticulum by PSORT program. This cDNA sequence did not show significant homology to any known sequences in Genbank database. RA39 is the first gene identified to be expressed specifically in tapetal cells at the meiosis stage of pollen mother cells from cereals.
利用cDNA减法杂交、差异杂交筛选和RACE等技术 ,从水稻 (OryzasativaL .ssp .japonica)中克隆了一个新的绒毡层特异性cDNA ,其编码基因被命名为RA39。该cDNA长 10 13bp ,编码由 2 98个氨基酸残基组成的多肽。RA39是一个单拷贝基因 ,在绒毡层细胞中特异性表达 ,在小孢子母细胞减数分裂期的绒毡层细胞中有较高的表达活性。用PSORT和PPSEARCH软件进行的结构分析揭示出RA39蛋白的N端是一个由 17个氨基酸残基组成的信号肽 ,该蛋白包含一个跨膜区和一个胞质尾区两个主要结构域以及多个蛋白激酶的磷酸化位点。
基金
国家自然科学基金 (3 0 170 5 72)~~
