白纹伊蚊经滞育卵传递登革Ⅱ型病毒的实验研究

Study on Diapause Eggs of Aedes albopictus Vertical Transmission of DEN-2

目的 证明登革Ⅱ型病毒能否在白纹伊蚊滞育卵内存活并传至子代。方法 采用逆转录 -聚合酶链反应检测病毒 ,C6 36细胞培养分离病毒。结果 能在白纹伊蚊滞育卵内检测到病毒 ,且滞育卵解除滞育后 ,子 1代幼虫和成蚊中均检测到病毒 ,总的批阳性率为 9.6 % ,子代最低感染率为 1∶2 94.3 ;第一个生殖营养周环子代未分离到病毒 ;第二、三生殖营养周环子代间最低感染率差异无显著性 ( χ2 =0 .0 1,P >0 .0 5 )。结论 试验证明登革Ⅱ型病毒能在白纹伊蚊滞育卵内存活并传至子代。

Objective To determine whether DEN-2 can survive in diapause eggs and be transmitted vertically to progeny of infected Aedes albopictus. Methods Reverse transcription-polymerase chain reaction (RT-PCR) combined with virus isolation in C6/36 cell were used for assay of the DEN-2 in diapause eggs and progeny of infected Aedes albopictus in labortary. Results DEN-2 was detected in diapause eggs and progeny after termintaed diapause.The total positive rate of pools was 9.6%, the minimum infection rate of the progeny was 1∶294.3;DEN-2 was not isolated from the progeny of the first oviposition cycle. The minimum infection rates were not significantly different for the second and third oviposition cycle( χ2=0.01,P>0.05). Conclusion DEN-2 can survive in diapause eggs and be transmitted vertically to progeny of Aedes albopictus.