猪主动脉组织工程瓣膜制备的初步研究

Primary study on tissue engineering porcine aortic valve preparation

目的 :初步探索猪主动脉组织工程瓣膜的制备方法。 方法 :经胰酶 EDTA、表面活性剂、核酸酶处理 ,去除猪主动脉瓣的细胞成分 ,测定瓣叶去细胞前、后的生物力学特性 ,并在其表面种植BAECs。 结果 :猪主动脉瓣膜中的细胞成分能完全被去除 ,获得完整无细胞的纤维网状支架 ,瓣叶去细胞前、后的断裂强度和断裂伸长率无明显变化 ,瓣膜近端存留的主动脉壁中的细胞一半被去除 ;种植的BAECs在去细胞瓣膜表面、主动脉内膜形成一连续的细胞层。 结论 :猪主动脉瓣膜去细胞后获得的纤维支架适宜血管内皮细胞的生长 。

Objective:To study the preparation of porcine heart aortic tissue engineering valve. Methods:The porcine aortic valve was acellularized with trypsin EDTA,Triton X 100,RNase and DNase treatment. Biomechanical characteristics of fresh valves and acellularized valves were tested,and the scaffold was seeded with BAECs for 3 times, and then cultured for another 5 d. Results:The acellularization procedure resulted in complete removal of the cellular components of valve while the construction of matrix was maintained, and half of cellular components of the aortic wall could be removed.The matrix could be successfully seeded with in vitro expanded BAECs,which formed a continuous monolayer on the surface. Conclusion:Acellularized porcine aortic valve may be applied as a scaffold to create tissue engineering heart valve.