摘要
目的 :探讨CO2 及N2 气腹对裸鼠腹腔恶性肿瘤细胞种植和生长的影响。 方法 :30只裸鼠分为 3组 ,即开腹组、CO2气腹组、N2 气腹组 ,每组 10只动物。术前 30min每只裸鼠腹腔注入 5× 10 5SHZ88细胞 ,术后 1h、4 8h取 3组裸鼠的前腹膜行透射电镜检查 ,术后 30d处死所有裸鼠 ,打开腹腔对比腹腔生长的肿瘤平均质量。 结果 :CO2 气腹组与N2 气腹组腹膜电镜下表现为间皮细胞变性、间皮细胞间连接断裂、基底膜裸露以及完整性破坏 ,开腹组腹膜的微观结构没有明显改变。术后30d开腹组腹腔生长的肿瘤平均质量为 (0 .736 8± 0 .1895 )g ,CO2 气腹组为 (1.786 1± 0 .0 399)g ,N2 气腹组为 (0 .82 6 0±0 .35 5 9) g ,其中CO2 气腹组腹腔生长的肿瘤平均质量与开腹组和N2 气腹组有显著性区别 (P <0 .0 1) ,开腹组与N2 气腹组腹腔生长的肿瘤的平均质量无显著性差别。 结论 :N2 气腹对裸鼠腹腔恶性肿瘤的生长无明显促进作用 ,CO2 气腹有明显促进作用 ,其主要原因可能在于CO2 气体本身。
Objective: To study the influence of carbon dioxide pneumoperitoneum (CP) on abdominal malignant tumor growth. Methods: Thirty nude rats were stratified into 3 groups( n =10). Group 1 was laparotomy, group 2 was CP, and group 3 was nitrogen pneumoperitoneum(NP). Thirty minutes before operation every rat was injected intraabdominally with 5×10 5 mammary adenocarcinoma cells (SHZ88 cell).One hour and 48 h after operation, morphologic alterations of the mesothelium was examined by transmission electron microscopy. Thirty days later, rats were killed to observe the difference of tumor growth in the 3 groups. Results: No significantly morphologic alterations of the mesothelium in group 1 were found. Group 2 and group 3 had the similar alterations such as mesothelial cell degeneration and broken connection between mesothelial cells and damaged underlying basal lamina.The average intraabdominal tumor weight in group 1 was (0.736 8±0.189 5) g, group 2 was (1.786 1±0.039 9) g, group 3 was (0.826 0±0.355 9) g.The average intraabdominal tumor weight of group 2 was significantly increased compared with that of group 3 and group 1( P <0.01).The average weight of group 3 abdominal tumor had no significant difference compared with that of group 1 ( P >0.05) Conclusion: CP significantly promotes abdominal tumor cells growth, the reason may be that carbon dioxide is not suitable for pneumoperitoneum.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第3期285-287,共3页
Academic Journal of Second Military Medical University
关键词
CO2气腹
N2气腹
腹膜肿瘤
carbon dioxide pneumoperitoneum
nitrogen pneumoperitoneum
peritoneal neoplasms