RET、Mucin1和Galectin-3在甲状腺良恶性肿瘤中的表达

Expression of RET,Mucin-1 and Galectin-3 in thyroid benign and malignant tumors

目的 :探讨滤泡源性甲状腺肿瘤及结节性甲状腺肿中RET、Mucin 1和Galectin 3蛋白的表达 ,寻找有助于诊断良恶性甲状腺肿瘤的标志物。 方法 :采用免疫组化S P法 ,检测 19例结节性甲状腺肿 ,5 4例腺瘤 (其中不典型腺瘤 19例 ) ,4 3例乳头状癌 (其中 11例乳头状微癌 ) ,16例滤泡性癌中上述 3种蛋白的表达情况。 结果 :良性病变 (结节性甲状腺肿和腺瘤 )与恶性病变 (乳头状癌和滤泡性癌 )中 3种蛋白的表达差异有极显著意义 (P <0 .0 1) ,而良恶性病变中的不同类型间的差异无显著性 (P >0 .0 5 )。 11例微癌患者中Galectin 3全部阳性 ,RET 8例阳性 ,Mucin 1阳性仅 3例 ;结节性甲状腺肿RET的表达与甲状腺癌比较虽无统计学意义 (P >0 .0 5 ) ,但前者为弱阳性 ,后者多为强阳性 ;腺瘤中不典型腺瘤Galectin 3的表达与其他腺瘤相比差异显著 (P <0 .0 5 ) ,与滤泡性癌相比差异无显著意义 (P >0 .0 5 ) ;部分腺瘤中见灶性Mucin 1和Galectin 3同时阳性。结论 :RET、Mucin 1和Galectin 3的同时检测可作为良恶性滤泡上皮性肿瘤鉴别诊断的指标 ,其中以Galectin 3的敏感性最佳 ,RET次之。Mucin 1高表达可能是去分化、浸润和转移的标志 ,Galectin 3阳性的不典型腺瘤是滤泡性腺瘤和滤泡性癌的交界性病变 ,良性腺瘤中Mucin 1?

Objective: To study the expression of RET,Mucin 1 and Galectin 3 proteins in thyroid follicular tumors and nodular goiter,looking for valuable diagnostic markers to distinguish the benign lesions from the malignant ones.Methods:By means of immunohistochemical streptavidin/peroxidase (S P) technique,19 cases of nodular goiters,54 cases of thyroid adenomas(including 19 cases of atypical adenomas),43 cases of papillary carcinomas(including 11 cases of papillary microcarcinomas) and 16 cases of follicular carcinomas were examed to detect the expression of RET,Mucin 1 and Galectin 3 proteins.Results:There were significant differences between benign and malignant lesions concerning the expression of 3 proteins( P <0.01).The positive rate of malignant groups was higher than that of benign ones,but there was no difference in each group itself( P >0.05).All (11 cases) microcarcinomas were positive of Galectin 3,8 cases of RET and 3 cases of Mucin 1.Although no statistical difference was found between nodular goiter and thyroid carcinoma( P >0.05),the former was chiefly weak positive reaction and the latter mostly showed strong ones.Expression of Galectin 3 in atypical adenoma was higher compared to adenoma ( P <0.05),and had no significant difference compared to carcinoma( P >0.05).Some adenomas was simultaneously positive of Mucin 1 and Galectin 3.Conclusion:Combined tests of RET,Mucin 1 and Galectin 3 may act to distinguish benign lesions from malignant tumors in clinical pathology.Galectin 3 is more sensitive than RET.The malignant follicular tumors with higher expression of Mucin 1 indicates dedifferentiation,invasion and metastasis.Atypical adenoma positive of Galectin 3 should be a borderline lesion between follicular adenoma and carcinoma.Mucin 1 and Galectin 3 positive simultaneously should take as the high risk state of thyroid carcinoma.