摘要
目的 :观察伽玛刀照射正常大鼠后急性期血脑屏障 (blood brainbarrier,BBB)通透性的改变。 方法 :选择正常大鼠右侧尾状核头部为照射靶点 ,动物按伽玛刀最大照射剂量分为 2 0、5 0、75、16 0Gy 4组 ,准直器直径为 4mm。于伽玛刀照射后12h至 14d内应用兔多抗清蛋白抗体行免疫组化染色半定量分析 ;并以硝酸镧作为示踪剂 ,应用透射电镜观察BBB的超微结构改变。 结果 :免疫组化法显示 16 0Gy和 75Gy剂量组于照射后 12h ,5 0Gy和 2 0Gy剂量组于照射后 1d靶区内出现血浆蛋白外渗 ;硝酸镧示踪电镜显示 16 0Gy、75Gy和 5 0Gy组于照射后 12h、2 0Gy组于照射后 1dBBB内皮细胞间的紧密连接开放。 75Gy、5 0Gy、2 0Gy组变化高峰期出现在照射后 3d ,而 7d后开始消散 ,16 0Gy组靶区阳性反应持续至照射后 14d。结论 :大鼠在伽玛刀照射后急性期存在BBB通透性改变 ,这种改变在 2 0～ 75Gy范围内具有自限性。
Objective: To study the effect of gamma irradiation on the permeability of the blood brain barrier(BBB) in rat brains. Methods:The right caudate nucleuses of Sprague Dawley rats were irradiated by OUR XGD gamma units.Maximum dosages of 20,50,75 and 160 Gy were given using a 4 mm collimator.Immunohistochemistry with antibody of serum albumin was used for detecting the extravasation of endogenous serum components.Ultrastructural changes of BBB were observed through injection of lanthanum nitrate into blood vessels. Results: Extravasation of albumin and BBB opening in the right caudate nucleuses were detected as early as 12 h after irradiation at 50,75 and 160 Gy,and were detected 1 d after irradiation at 20 Gy.Immunoreactivity and emematous water reached their maximum after 3 d, gradually decreased during the following few days,and disappeared by day 7(20,50 Gy) or day 14(75 Gy).Irradiation at 160 Gy elicited persistent extravasation of albumin and BBB opening for 14 d. Conclusion: These are transient impairments to BBB after irradiation at 20,50,75 Gy,and persistent impairments after irradiaton at 160 Gy.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第3期314-317,共4页
Academic Journal of Second Military Medical University
基金
全军"九五"医药科研规划重点课题(0 1MA0 5 1)
