白肋烟TN86晾制期PPO、POD活性及总酚、类黄酮含量动态研究

Development of PPO,POD activity and contains of total phenols,flavonoid of TN86 in air-curing stage

TN86晾制期多酚氧化酶(PPO)活性是随晾制进程而增加的双峰曲线,第一峰域矮而小、时值短、在0～6d,第二峰域高而宽大、时值长、在6～21d,酶的活动主要在6d后烟叶的变褐期;过氧化物酶(POD)活性则是随晾制进程而变动的双峰曲线,3d与6d时为高峰期,且第一峰值比第二峰值高,其活动主要在烟叶的凋萎期,其次才是变黄期,PPO与POD活性均随叶位升高而增大。总酚与类黄酮的含量都随晾制进程而下降,随叶位升高而增添,其含量变化稍滞后于PPO的活性动态,证实二者密切相关;晾制后各叶位总酚含量为0.35%～0.58%,属优质白肋烟相应指标,证明本研究的晾制技术规范切实可行,保持了白肋烟风格。

Two-humped curve of the PPO activity of burley tobacco TN86is observed in the process of air-cur-ing.The first peak is lower and small,whose time value is in0-6d.The second peak scope is bounty,and time value is longer,which is in6-21d,the activity of PPO is mainly in6d leaf browning stage.The activ-ty is two-humped curve as changing of air-curing process.Its high peak is in3d and6d respectively.The first peak,which is in wilting stage,is higher than the second peak value in yellowing.The activity of PPO,POD will increase with the stalk position hoisting.The contains of total phenols,flavonoid will decrease as air-curing goes on,and increase as leaves position goes up,whose containing change is a little later than activity change of PPO,which confirm the relationship of the two.The total phenols of all stalk posi-tions are in the range of0.35%-0.58%after air-curing,which belong to good-quality burley tobacco scope.It is practical for this air-curing study.