中国玉米新品种DNA指纹库建立系列研究 Ⅰ.玉米品种纯度及真伪鉴定中SSR技术标准实验体系的建立

Series of Research on Establishing DNA Fingerprinting Pool of Chinese New Maize Cultivars Ⅰ.The Establishment of a Standard SSR System Fitting for Maize Cultivars' Identification

本研究从DNA提取、PCR扩增、电泳检测等环节对SSR技术进行了优化,最终建立一套完善的适用于玉米品种鉴定的SSR标准体系。并以玉米杂交种农大108的纯度鉴定为例,探索了该体系在玉米杂交种鉴定中实际应用的可行性。

SSR technique was optimized from DNA extraction,PCR procedure and electrophoresis detection.A standard SSR system fitting for maize hybrid identification was established finally.The hybrid purity identification of Nongda108was used as an example to explore the possibility of application of SSR technique on maize hybrid identi-fication.