摘要
本研究从DNA提取、PCR扩增、电泳检测等环节对SSR技术进行了优化,最终建立一套完善的适用于玉米品种鉴定的SSR标准体系。并以玉米杂交种农大108的纯度鉴定为例,探索了该体系在玉米杂交种鉴定中实际应用的可行性。
SSR technique was optimized from DNA extraction,PCR procedure and electrophoresis detection.A standard SSR system fitting for maize hybrid identification was established finally.The hybrid purity identification of Nongda108was used as an example to explore the possibility of application of SSR technique on maize hybrid identi-fication.
出处
《玉米科学》
CAS
CSCD
2003年第1期3-6,共4页
Journal of Maize Sciences
基金
农业部及北京市重点科技项目。