摘要
采用PCR -RFLP技术 ,TaqI酶切、分析了 38头份样品的小尾寒羊FSHR基因 5′端转录启动调控区序列。检测结果未发现多态性 ,在牛FSHR基因 5′端 - 387→ - 384位点 ,中国西门塔尔牛表现TaqI酶切多态的位点 ,小尾寒羊表现为无TaqI酶切序列的B等位基因类型 ,所有个体均为BB型。由于小尾寒羊的双羔率在10 0 %以上 ,通过比较表明
s: Polymorphism of 5′-flanking region of the Follicle-stimulating Hormone Receptor(FSHR) Gene of Chinese fine-tail Han Sheep was analysized with TaqⅠ by PCR-RFLP. At the locus that is a polymorphic locus to Chinese Simmental, Only one type of DNA sequence similar to allele-B type of Chinese Simmental was found for all of 38 samples of fine-tail Han Sheep. Comparative study suggested that DNA sequence similar to allele B may be favourable to animal's twinning rate.
出处
《草食家畜》
2003年第1期58-60,共3页
Grass-Feeding Livestock
基金
湛江师范学院专项资金资助
关键词
小尾寒羊
促卵泡素受体基因
转录启动调控区
双胎率
FSHR gene
Chinese fine-tail han sheep
twinning rate
region of promoter and triscriptional regulator