小尾寒羊促卵泡素受体基因5′端序列的TaqI酶切多态性分析

Analysis of polymorphism of 5′-flanking regionof FSHR gene of Chinese fine-tail han sheep by TaqⅠ

采用PCR -RFLP技术 ,TaqI酶切、分析了 38头份样品的小尾寒羊FSHR基因 5′端转录启动调控区序列。检测结果未发现多态性 ,在牛FSHR基因 5′端 - 387→ - 384位点 ,中国西门塔尔牛表现TaqI酶切多态的位点 ,小尾寒羊表现为无TaqI酶切序列的B等位基因类型 ,所有个体均为BB型。由于小尾寒羊的双羔率在10 0 %以上 ,通过比较表明

s: Polymorphism of 5′-flanking region of the Follicle-stimulating Hormone Receptor(FSHR) Gene of Chinese fine-tail Han Sheep was analysized with TaqⅠ by PCR-RFLP. At the locus that is a polymorphic locus to Chinese Simmental, Only one type of DNA sequence similar to allele-B type of Chinese Simmental was found for all of 38 samples of fine-tail Han Sheep. Comparative study suggested that DNA sequence similar to allele B may be favourable to animal's twinning rate.