摘要
目的 :观察益骨胶囊对体外培养成骨细胞增殖和分泌胰岛素样生长因子Ⅰ (IGF Ⅰ )的影响 ,探讨该方的作用机制。方法 :分离、培养新生大鼠的原代成骨细胞 ,采用MTT法测定含药血清作用后的成骨细胞增殖和分泌IGF Ⅰ的量。结果 :益骨胶囊含药血清中、高剂量组成骨细胞增殖和分泌IGF Ⅰ的量均明显高于对照血清组 (P <0 .0 1)。结论 :益骨胶囊具有促成骨细胞增殖和分泌IGF Ⅰ的作用 。
Objective:The effects of Yigu capsule on proliferation and insulin like growth factor Ⅰ(IGF Ⅰ) secretion by osteoblasts were studied in order to further explore the mechanism of preventing and treating osteoporosis. Method:The newborn rat osteoblasts were cultured with different drug containing serum concentration in Yigu capsule, Methyl thiazolyl tetrazolium method was used to observe proliferation of osteoblasts andenzyme linked immunosorbent assay was used to oberserve IGF Ⅰ secretion. Result: Proliferation and IGF Ⅰ secretion of osteoblasts with middle and high drug containing serum concentration were more than those of the control groups respectively(P<0.01). Conclusion:The effects of promoting proliferation and IGF Ⅰ secretion of osteoblasts are one of the mechanism of Yigu capsule in preventing and treating osteoporosis.
出处
《安徽中医学院学报》
CAS
2003年第2期45-47,共3页
Journal of Anhui Traditional Chinese Medical College
基金
广东省自然科学基金资助项目 (990 4 75 )
