无血清培养人肝癌细胞增生率的变化和bFGF在细胞中的表达

Dynamic Changes of Cell Proliferative Rates and Expression of Basic Fibroblasts Growth Factor in Serum-free Culture of Human Hepatocellular Carcinoma in Vitro

采用无血清培养体系 ,研究肿瘤细胞自分泌调控机制。将人肝癌细胞培养在无血清无外源分裂因子培养基中 ,采用细胞原位计数法和免疫组织化学染色法 ,观察不同时相人肝癌细胞增生率的变化以及碱性成纤维细胞生长因子 (bFGF)的表达情况。结果 :人肝癌细胞生长良好 ,其增生速度与其分泌的bFGF量成正比。提示 :肝癌细胞在无外源生长因子的条件下通过分泌bFGF ,以自分泌和旁分泌的方式作用于自身和相邻细胞 ,促进细胞生长和增生。

The objective was to study the mechanism of short range modulation of carcinoma cells in serum free culture medium. The cell measurement in situ and immunohistochemical staining were employed to observe the dynamic changes of cell proliferative rate and the expression of basic fibroblasts growth factor (bFGF) of human hepatocellular cells that were cultured in serum free and mitogen free medium. Results: With the changes of cell proliferative rate, the dynamic changes of expression of bFGF at different phases were found. When the proliferative rate was increased, the expressions of bFGF increased strongly, and vice versa. These suggest that the autocrine and paracrine growth factors play an important role in short range modulation of cell proliferation.