纳豆激酶粗制液对家兔溶血栓作用及其机制研究

STUDIES ON FIBRINOLYTIC FUNCTION OF NATTOKINASE AND ITS MECHANISM

目的 : 观察纳豆激酶粗酶液体外、体内溶解血栓的作用。方法 : 2 0 % Fe Cl3制造兔颈动脉血栓模型 ;1 8只日本大耳兔 ,随机分为 3组 ,即十二指肠肠腔注射纳豆激酶 (Nattokinase,NK)高、低剂量实验组 (分别 45 0 0 U/ kg、2 5 0 0 U/ kg)、生理盐水模型对照组。结果 : 体外试验表明 ,纳豆菌株 B.N.1 2液体发酵粗酶液作用血凝块 1 0 h、2 2 h后 ,溶解率分别为 78.2 3 %、98.1 6% ,对照组分别为 2 1 .43 %、2 3 .91 % (P<0 .0 5 ,P<0 .0 1 ) ;动物试验表明 ,与对照组相比 ,NK高剂量组注射 1 .0 h、1 .5 h后 ,凝血时间 (coagulation time,CT) (P<0 .0 5 )、凝血酶原时间 (prothrombintime,PT) (P<0 .0 5 )、凝血酶时间 (thrombin time,TT) (P<0 .0 1 )和活化的部分凝血活酶时间(activiated partial thrombopastin time,APTT) (P<0 .0 5 )均显著延长 ,注射 2 h后优球蛋白溶解时间 (euglobulinlysis time,ELT)和纤维蛋白原 (fibrinogen,FIG)含量显著降低 (P<0 .0 1、P<0 .0 5 ) ,纤维蛋白降解产物 (fibrinogen degradation products,FDP)含量明显增高 (P<0 .0 5 ) ,血浆D-二聚体 (D-dimer)呈阳性 ,病理组织切片表明肺静脉、肾小球毛细血管中无明显血栓 ,只有少量红细胞聚积 ;低剂量组 CT、PT和

Objective: To study the fibrinolytic effects of activity of nattokinase (NK) in different natto strains in vitro and in vivo. Methods: Activity of NK was measured by clot liquefaction time (CLT). The model of carotid thrombosis was induced by 20% FeCl 3 stimulation. 18 rabbits were divided into 3 groups : high, low NK dose (4 500 U/kg, 2 500 U/kg respectively) groups and 0.9% NaCl control group. All were injected through duodenum. Results: The activity of NK made from submerged fermentation by B.N.12 was 338 U/ml while that from solid fermentation was 296 U/g, and that by B.N.10 was 272 U/ml and 346 U/g, respectively. After treatment in vitro 10,22 h later by NK from submerged fermentation of B.N.12, the blood clot was significantly dissolved 78.23%?98.16% respectively, but that of control group was 21.43%?23.91% respectively. The experiment in vivo indicated that high dose group could significantly prolong CT?PT?TT?APTT (P<0.01,P<0.05), reduce ELT(P<0.01), decrease the content of FIG (P<0.05), increase the content of FDP (P<0.05), the venous thrombus in lung and kidney was dissolved totally or partly as observed by pathological section . The low dose group could reduce ELT (P<0.05),prolong TT (P<0.05), increase the content of FDP (P<0.05), but there was no significant difference in CT?PT?APTT and FIG compared with control group and with that before injection. D dimer of all experimental groups was positive. Conclusion: Both thrombolytic effects of NK in vitro and in vivo were significant. One of the mechanisms may be associated with enhancing fibrinolysis and anticoagulation activity.spectively.Aftertreatmentin vitro1 0 ,2