输血后肝炎患者血清中丙型肝炎病毒抗体及核酸对照检测的临床意义

HCV Infection in Patients With non-A, non-B Hepatitis Detected by Polymerase Chain Reaction and Serum Anti-HCV

1989年Choo等首先成功地从受感染的黑猩猩血液标本中克隆了丙型肝炎病毒(HCV)cDNA,尔后相继建立的血清HCV异性抗体(抗-HCV)试剂盒和检测HCV核酸(HCVRNA)的聚合酶链反应(Polymerase Chain Reaction, PCR,使对丙型肝炎病毒及丙型肝炎的研究取得了突破性进展。目前国内外已广泛采用检测血清中抗-HCV来确定输血后这一病毒的染感,但由于抗-HCV在血清中出现较晚,难以早期发现病人,所以输血后肝炎中丙型肝炎病毒的确切感染率尚不清楚。为此我们检测了35例输血后肝炎患者血清中的抗-HCV和HCV-RNA。

To study the diagnosis of hepatitis C virus (HCV) infection in non A, non-B posttransfusion hepatitis and the corelation between HCV-RNA and anti-ACV. HCV-RNA was tested by reversely transcribed and nested polymerase chain reaction (RT-nPCR) and anti-HCV by ELISA. In 35 cases of posttransfusion hepatitis. HCV-RNA was detected in 20 (57.14%) of 35. Of 20 anti-HCV positive patients, 14 were HCV-RNA positive and 15 anti-HCV negative, there were 11 cases with HCV-RNA positive. Hence, anti-HCV poitive is most likely accompanied by the existence of viremia. In otner words, most of posttransfusion hepatitis patients with negative anti-HCV were still caused by HCV.