HLA—D抗原的相容性以及封闭因子与习惯性流产的关系的实验研究

Research on the Relation of Histocompatibility of HLA-D Antigen and Blocking Factors to Habitual Abortion

运用单向混合淋巴细胞培养的方法分别检测了10例习惯性流产史船娠夫妇间、流产史孕妇和无关第三者(男性)间及9例正常妊娠夫妇间的HLA-D抗原的相容性。结果发现流产史夫妇间的HLA-D抗原相容性大于流产史孕妇和无关第三者(男性)之间及正常夫妇间的HLA-D抗原相容性(P<0.01)。提示夫妇间的HLA-D抗原相容性增大可能是导致习惯性流产的原因之一。同时,在反应体系中分别加入不同孕妇血浆以观察其对单向混合淋巴细胞培养(单向MLC)的影响时发现,流产史孕妇血浆对单向MLC不仅无抑制作用,反而有刺激作用,而正常孕妇血浆对单向MLC有抑制作用。推测正常孕妇血浆中存在特异和/或非特异的封闭因子,而流产史孕妇血浆中缺乏特异和/或非特异的封闭因子。

In this research, the method of one-way mixed ilymphocyte culture(MLC) was used to ascertain the histocompatibility of HLA-D autigen of 10 abortioncouples, that of 9 control-couples with no abortion history, and that of the control group of abortion women with unrelative males. The results of oneway MLCs indicated that the degree of HLA-D antigen sharing in the recurrent abortion couples was greater than that in the control groups(P<0.01). It is suggested 'that the histocompatibility of HLA-D antigen between a woman and her husband might be one of the factors of habitual abortion. In the meantime, in order to observe the influence of pregnant plasma on one-way MLCs, the plasma of pregnant abortion-woman and control-woman were respectively added into one-way MLCs. It was found that the plasma of the pregnant abortion-woman had a stimulative effect on one-way MLCs. On the contrary, the plasma of the pregnant control-woman exerted a suppressive effect on one-way MLCs. It is believed that specific and/or non-specific factors might exist in 'the plasma of the pregnant control-woman while in the plasma of the pregnant abortion-woman there might not exis't these blocking factors.