新基因ZNF325在人类早期胚胎中的表达

Studies on Expression of a Novel Gene ZNF325 in Human Embr oynic Development

锌指蛋白基因家族是人类最大的基因家族之一 ,目前已知的很多转录调控因子都是锌指蛋白成员[1 ] .初步克隆了一个与RBAK基因有着高度同源性的人类C2 H2 型锌指蛋白新基因 ,经国际基因命名委员会批准命名为ZNF32 5 .此基因位于染色体 7p2 2 ,长 2 6 97个碱基 ,含 5个外显子 ,在基因组DNA上长 15kb .它编码的蛋白质全长46 2个氨基酸 ,含 15个C2 H2 型的锌指 .NorthernBolt分析表明该基因在人类早期胚胎的各个组织中普遍表达 ,在肺和脑中的表达水平最强 ,但在肝中的表达随着胚胎的发育而逐渐减少 .它的结构和表达特征预示着它编码的是一个具DNA结合功能的转录调控因子 .

Zinc finger protein family is one of the largest families an d lots of them are the transcription regulators.We found a novel h uman C 2H 2 type zinc finger protein named ZNF325 that has high homology w ith RBAK and located at 7p22. Its mRNA is 2?697?bp with 5 exons that span 15? kb in geno mics . It contains 462 amino acids, containing 15 C 2H 2 zinc fingers that show hig h ho mology with RBAK. The gene is wildly expressed in the various tissues of human e mbryos, especially in the lung and brain but its expression reduced in the liver following the development. The structure and expression pattern implies it migh t be a DNA binding transcription regulator.