损伤小脑效应、去大脑僵直"实验方法的改进

Improvement of Experimental Methods on Injuring Cerebellum and Decerebrate Rigidity

为了探索新的、高成功率的损伤小脑效应和去大脑僵直的实验方法,并使2个独立的实验合并进行,笔者采用非麻醉、非开颅的手段,对小鼠小脑一侧进行损伤,并在此基础上,于小鼠人字缝前0.5～1mm处横断脑干,去除大脑.结果显示:小鼠一侧小脑的效应100%显现;绝大多数实验鼠表现出去大脑僵直的效应,实验成功率高达75%.合并2个独立的实验以及使用小鼠代替传统的家兔和猫进行去大脑僵直实验是可行的,其操作简单,重复性好,动物价格低廉,极易于在学生实验中开展.

To search a new, high successful experimental method about injuring cerebellum and decerebrate rigidity and to merge two independent experiments into one. The authors made an injury on one side of mouse's cerebellum by nonanesthesia and noncraniotomy, and basing on this,cut across the brain stem at 0.5-1 cm in front of the mouse's lambdoid suture to remove the cerebrum. The results showed that injuring cerebellum had 100 percent of effect on mice, whereas removing the cerebrum had the decerebrate rigidity effect on most of the experimental mice, with the successful probability up to 75 percent. Thus the authors thought that it was feasible to merge two independent experiments into one and to replace traditional rabbits or cats with mice for conducting the experiment of decerebrate rigidity, and this method was simple in operation, better in repeatability,easy carried out in the course of experimcnt by students; meantime the animals required by the experiment was cheap in price.