摘要
比较了酵母菌发酵生产谷胱甘肽 (GSH)的几种补料分批培养方式。实验发现补料可以明显地促进酵母菌的生长和谷胱甘肽的合成 ,同时还发现不同的补料方式对发酵液中的菌体浓度和GSH浓度有不同的影响 :采用指数流加方式可获得极高的菌体浓度 ,但菌体中的GSH浓度较低 ;而采用恒 -pH补料分批培养既可以达到较高菌体浓度、菌体中又含有较高的GSH含量 ,因此 ,其总的GSH产量最高 ,可达到 977.8mg L。
Several feeding fed-batch culture methods for the production of glutathione (GSH) by Saccharomyces cerevisiae were compared. The results showed that the feeding could obviously promote the growth of S.cerevisiae and the synthesis of glutathione, and different feeding methods had different influences on the biomass and GSH in the broth. The highest biomass could obtain by exponential feeding, but the concentration of GSH was lower; higher biomass and higher GSH could be obtained by pH-stat fed-batch culture, the total concentration of GSH (977.8mg/L) was highest.
出处
《工业微生物》
CAS
CSCD
北大核心
2003年第1期19-22,共4页
Industrial Microbiology
基金
中国博士后科学基金 (第 2 7批 )资助项目
关键词
补料分批培养
酵母菌
谷胱甘肽
生产
fed-batch culture
Saccharomyces cerevisiae
glutathione
