摘要
以黄瓜卷须为材料,对黄瓜染色体制片中的一些主要参数和方法进行了研究,建立了黄瓜染色体制片的系统操作规程。研究发现,黄瓜染色中心直径和异染色质数目与植株倍性呈正相关,可作为黄瓜倍性鉴定的间接指标,其中单倍体染色中心直径平均为5 1μm,双单倍体为13 3μm,单倍体异染色质数目平均5 0,双单倍体为11 9。
Main parameters and methods of chromosome preparing with wall degradation hypotonic technique in cucumber were studied,and a suitable method was established.The results indicated that there was a evident correlationship between chromocenter diameter,heterochromatin number and plant ploidy level.The chromocenter diameter and heterochromatin number of haploid and double haploid are 5.1,5.0 μm and 13.3,11.9 μm respectively.
出处
《华北农学报》
CSCD
北大核心
2003年第1期72-74,共3页
Acta Agriculturae Boreali-Sinica
基金
天津市重点攻关项目(993122511-3)
关键词
黄瓜
染色体制片
倍性
切片技术
Cucumber
Chromosome
Ploidy
Appraisal
Section cutting
