摘要
目的:为研究肿瘤的多药耐药现象,应用胃癌耐药相关的单克隆抗体MGr1筛选肿瘤耐药细胞表达文库获得的一个稳定阳性克隆的cDNA片段MGr1-Ag1,检测MGr1-Ag1在胃癌组织中的分布及表达水平.方法:利用原位杂交技术,对MGr1-Ag1mRNA在胃癌组织中的分布进行了检测,以了解其在胃癌中的表达情况及意义.结果:MGr1-Ag1mRNA定位在胃腺体细胞的胞质中,为蓝色小颗粒,呈弥漫性分布.MGr1-Ag1mRNA在SGC7901/VCR阳性信号明显高于SGC7901,胃癌与癌旁组织相比,MGr1-Ag1mRNA的阳性表达率均有显著差异(P<0.05).MGr1-Ag1mRNA在胃癌(50.00%)、癌旁组织(71.42%)、高分化腺癌(68.75%)和中分化腺癌组织(57.14%)中的阳性表达率无显著差别,但高分化组和中分化组都与低分化腺癌组织(10.00%)差别显著(P<0.05).此外,MGr1-Ag1mRNA还分布于小肠及大肠腺体内,因例数偏少,未做统计.结论:MGr1-Ag1与胃癌的分化程度有关,随着组织的分化程度增高,MGr1-Ag1mRNA的阳性表达率增加.
AIM:To analyze the distribution and significance of the pos- sible cDNA fragments encoding MGr1-Ag1 in human gastric cancer. METHODS:We investigated MGr1-Ag1 expression in gas- tric cancer using in situ hybridization (ISH) techniques in 42 cases of gastric carcinomas in cryostatic sections collected from Xijing Hospital. RESULTS:MGr1-Ag1 mRNA was positive in cytoplasm of gastric glandulous epithelia with intensive staining in 50.00 % (21/42) cases with gastric cancer, and 71.42 % (30/42) cases in paracancerous gastric tissues.Eleven of 16 (68.75 %) cases were well differentiated, 8/14(57.14 %) cases were moder- ately differentiated and 1/10(10.00 %) cases were poorly differentiated. The positive rate of MGr1-Ag1 mRNA in gas- tric cancer was significantly different from that in paracancerous gastric tissues (71.42 %). There was no sig- nificant difference in positive rate of MGr1-Ag1 mRNA among normal, paracancerous gastric tissues, well and moderately differentiated tissues, but the positive rate in the poorlydifferentiated tissues was low, being significantly different from that in well or moderately differentiated tissues. CONCLUSION:MGr1-Ag1 mRNA is mainly localized in epi- thelial cells, and the level of its expression is correlated with the tumor differentiation.
出处
《世界华人消化杂志》
CAS
2003年第1期18-21,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金资助课题
No.30030140和30024002~~
