碱性成纤维细胞生长因子在胃溃疡愈合中的表达及意义

Expression of basic fibroblast growth factor (bFGF) in healing human gastric ulcer

目的:探讨人胃溃疡愈合过程中碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)的变化及其作用.方法:采用免疫组织化学法,对正常胃黏膜(20例)、胃溃疡活动期(24例)、愈合期(26例)和瘢痕期(20例)组织的bFGF的表达进行定位观察和图像半定量分析.结果:bFGF在正常胃黏膜呈弱阳性表达,在胃溃疡急性期呈阳性表达,在愈合期和瘢痕期呈强阳性表达.阳性信号主要位于腺上皮、细胞外基质、成纤维细胞和内皮细胞中.bFGF平均光密度值和面密度值在胃溃疡活动期、愈合期、瘢痕期与正常胃黏膜相比均有显著性差异(0.247±0.042&0.321±0.096&0.296±0.048vs0.125±0.062,P<0.05;0.131±0.024&0.165±0.031&0.162±0.028vs0.081±0.008,P<0.05),在愈合期及瘢痕期组织与活动期溃疡相比也有显著性差异(0.321±0.096&0.296±0.048vs0.247±0.042,P<0.05;0.165±0.031&0.162±0.028vs0.131±0.024,P<0.05).结论:在人胃溃疡愈合过程中,存在着内源性bFGF由弱到强的演变,说明bFGF与胃溃疡愈合密切相关.临床上合理应用bFGF有助于消化性溃疡愈合.

AIM:To investigate changes of basic fibroblast growth factor (bFGF) in the healing of human gastric ulcer. METHODS:The expression of basic fibroblast growth factor (bFGF) in human gastric mucosa: normal (20 cases), active stage of gastric ulcer (24 cases), healing stage of gastric ulcer (26 cases) and scarring stage of gastric ulcer (20 cases) was detected by immunohistochemical methods and com- puterized image analysis. RESULTS:The expression intensity of bFGF was different among normal gastric mucosa and mucosa at different stages of gastric ulcer. The expression in normal gastric mucosa was weakly positive, at the active stage of gastric ulcer was positive, at the healing stage and scarring stage was strongly positive. The positive signal of bFGF was localized in granu- lation tissues, extracellular matrix (ECM), fibroblasts and endothelial cells. There were significant differences in aver- age integral optic density and area density among different stages of gastric ulcer and normal gastric mucosa (0.247±0.042, 0.321±0.096, 0.296±0.048 vs 0.125±0.062, P<0.05; 0.131±0.024, 0.165±0.031, 0.162±0.028 vs 0.081±0.008, P <0.05) They were significantly higher at the healing stage and scarring stage than that at the active stage in average integral optic density and area density ( 0.321±0.096, 0.296±0.048 vs 0.247±0.042, P <0.05; 0.165±0.031, 0.162±0.028 vs 0.131±0.024, P<0.05). CONCLUSION:The changes of bFGF were closely related to the healing of human gastric ulcer, and rational use of bFGF can promote the healing of gastric ulcer.