乳腺癌中FHIT基因缺失与人乳头状瘤病毒感染相关性的研究

Fragile histidine triad gene deletion in breast cancer and its correlation with human papillomavirus infection

目的探讨乳腺癌组织中FHIT基因缺失的临床意义和人乳头状瘤病毒 (humanpapillo mavirus ,HPV)感染的关系。方法采用RT PCR检测了 38例乳腺癌及 10例正常乳腺组织中FHIT基因缺失情况 ,并用PCR技术检测HPV DNA片段。结果乳腺癌组织中FHIT基因缺失率及HPV DNA片段检出率分别为 6 3 1% ( 2 4/38)、31 6 % ( 12 /38) ,正常组织未检出FHIT基因缺失 ( χ2 =12 6 32 ,P <0 0 1;χ2 =4 2 11,P <0 0 5 )。FHIT基因缺失与乳腺癌组织分化程度、临床分期、淋巴结转移、远处转移密切相关 ;与 3年生存率呈负相关。在 12例HPV阳性组织中有 11例 ( 91 7% )发生FHIT基因缺失 ( χ2 =6 12 6 ,P <0 0 5 )。结论FHIT基因的缺失与乳腺癌发生发展密切相关 ,可作为判断乳腺癌恶性程度及预后的指标。HPV的感染与乳腺癌的发生有关。

Objective To investigate the deletion of fragile histidine triad (FHIT) gene in breast cancer and explore its relation with human papillomavirus(HPV) infection. Methods Thirty-eight breast cancer specimens and ten normal breast tissues were examined for abnormalities of FHIT gene by RT-PCR and fragment of HPV-DNA by PCR. Results The positive rates of FHIT gene deletion and HPV-DNA fragment were 63.1%(24/38) and 31.6%(12/38) respectively, while they were not detected in normal tissues (χ 2=12.632,P<0.01;χ 2=4.211,P<0.05). The deletion of FHIT gene in breast cancer was significantly correlated with pathological grading, lymph node metastasis, clinical stage and distant metastasis of breast cancer. There was an inverse relation with three-year survival rate. In all 12 cases of HPV infection, there were 11 cases in which the deletion of FHIT gene was found (χ 2=6.126, P<0.05). Conclusions The deletion of FHIT gene is correlated with the carcinogenesis and progress of breast tumor and thus it could be used as a mark of malignant potentiality and a predictor of postoperative prognosis. HPV infection contributes to breast canceration.