左旋多巴和多巴胺对中脑原代细胞的毒性作用

Toxicity of levodopa and dopamine on cultured rat mesencephalic cells

目的 :观察左旋多巴和DA对中脑原代培养细胞的毒性作用。 方法 :采用大鼠胚胎中脑原代细胞培养法 ,运用TH免疫荧光染色和 [3H]DA摄取率检测DA能神经元的存活数和功能 ;GFAP免疫荧光染色检测星形胶质细胞的存活数 ;以及MTT检测非DA能神经元的存活数。 结果 :左旋多巴或DA处理后的TH阳性和GFAP阳性细胞数以及细胞存活率均显著低于加药前基数 ,且呈剂量依赖性 ;同时残存细胞体积变小 ,突起减少、变短或断裂。TH阳性细胞和GFAP阳性细胞比非DA能神经元更易受损。结论

Objective: To observe the toxic effects of levodopa and dopamine on cultured rat mesencephalic cells.Methods: Survival and function of DAergic neurons were assessed by immunohistochemical measurement of the number of tyrosine hydroxylase (TH) positive cells and by isotope detection of \ DA uptake. The survival of astrocytes was measured by immunohistochemical detection of the number of GFAP positive cells. The survival of non DAergic neurons was detected by MTT assay. Results: The number of TH positive cells and GFAP positive cells as well as the viability of mesencephalic cells were significantly lower in levodopa or dopamine treated groups in a dose dependent manner. The remaining neurons became smaller and the process of axon was reduced, shortened or broken. TH and GFAP positive cells were more severely affected than non DAergic neurons after exposure to low dose of levodopa or dopamine. Conclusion: Levodopa and dopamine were neurotoxic to both DAergic and non DAergic neurons in mesencephalic neuronal enriched cultures from rat embryos. \[