家兔原位性肾炎发展过程中血浆和肾皮质TXB_2和6-Keto-PGF1_α的观察

The Observation of TXB_2 and 6-Keto-PGF_α in Plasma and Renal Cortex in the Course of Nephritis in Situ in Rabbits

观察了家兔原位性肾炎发生发展过程中血浆和肾皮质TXB_2和6-Keto-PGF_1α的变化。结果表明,随着肾炎病变的发展,尿蛋白明显增多,血肌酐增加,血浆TXB_2和6-Keto-PGF_1α明显增多,尤以TXB_2显著;当病变趋向恢复时,TXB_2有所减少而6-Keto-PGF_1α增加。当病变趋向恢复时,肾皮质两种前列腺素与正常相比明显增多,并以TXB_2显著。研究提示,原位性肾炎家兔血浆和肾皮质中TXB_2和6-Keto-PGF_1α发生明显改变,其改变程度在肾炎发展各阶段有显著不同。

Serum sickness nephritis was induced in New Zealand white rabbits by intravenous injections of cationic bovine serum albumin(C-BSA) . The concentration of Scr and urinary protein was determined, TXB_2 and 6-Keto-PGF_1α were analyzed with radioimmunoassay and pathological changes of renal tissues were examined. The results showed that urinary protein and Scr increased, TXB_2 and 6-Keto-PGF_1α in plasma, especially TXB_2, also increased significantly in progress of nephritis. TXB_2 in plasma decreased slightly but still maintained at high level, while 6-Keto-PGF_1α kept going up when the pathological changes of nephritis went towards recovery. TXB_2 and 6-Keto-PGF_1α in renal cortex of nephritis animal were higher than that in the normal renal cortex. Our experiments suggest that there are great changes of TXA_2 and PGI_2 in rabbits with nephritis in situ, and these changes may link closely with the whole course, develop and progress as well as recovery, of nephritis.