以啤酒洗槽废水为主料产朊圆酵母液体发酵的研究

Study on Submerged Culture of Torula Utilis in Waste Water from Washing Beer Tank

以啤酒洗槽废水为主料通过单因素优选和正交试验法对产朊圆酵母进行液体发酵培养基的筛选与发酵条件的确定,经方差分析结果最佳培养基为2°Be糖度的啤酒洗槽废水,豆饼粉0.5%,葡萄糖2%,MgSO_4 7H_2O 0.05%,KH_2PO_4 0.1%,最佳发酵条件为时间18h,温度28℃,转数150r/min,装量100ml/250mL三角瓶,pH5.0,干菌体积累量为20.12g/L。

It was proved by single facter optimization and orthogonal test that the optimum medium for submerged culture of Torula utilis were 2°Be waste water from washing beer tank, added 0. 5% soybean cake flour, 2% glucose, MgSO4 7H2O 0. 05%, KH2PO40. 1%, and that the proper submerged culture conditions were 28℃, 18h, 150r/min, pH5.0 and 100ml liquid medium in the 250mL bottle, 20.12g dry yeast per liter liquid medium was gained.