二氧化硫吸入对小鼠9种脏器GSH和GSH/GSSG的影响

Effect of SO_2 inhalation on the levels of GSH and GSH/GSSG in 9 organs of mice

为研究二氧化硫 (SO2 )吸入后小鼠多种脏器内抗氧化物质谷胱甘肽 (GSH)和抗氧化系统GSH GSSG(GSSG为氧化型谷胱甘肽 )的变化 ,采用SO2 动态吸入染毒技术 ,使昆明小鼠吸入不同浓度SO2 后 ,用分光光度法测定不同脏器GSH含量 ,并计算GSH GSSG比值。结果表明 :(1)在SO2 浓度为 2 2mg m3时 ,雌、雄鼠所试脏器GSH水平和GSH GSSG均无显著变化 ;(2 )在SO2 浓度为 64mg m3时 ,雌鼠GSH水平和GSH GSSG变化不大 ,而雄鼠肺、肾、脑、胃、睾丸中GSH水平显著降低 ;(3 )在SO2 浓度为 148mg m3时 ,雌、雄鼠各脏器GSH水平和GSH GSSG均有下降 ,其中GSH在雄鼠肝、肺、肾、心、脑、胃、小肠、睾丸和雌鼠肝、肺、肾、小肠中下降显著 ,GSH GSSG在雄鼠肝、肾、小肠、睾丸和雌鼠肝、肺、肾、脑、胃、小肠中下降显著。以上结果说明一定浓度的SO2 吸入不仅影响小鼠呼吸器官 ,而且对多种脏器都有作用 ,同时显示雌、雄小鼠对SO2 的敏感性不同。GSH含量减少和GSH GSSG下降提示SO2 的毒作用与其降低体内抗氧化物质水平。

By spectrophotometric method, the level of non enzymatic antioxidant glutathione (GSH) and the ratio of GSH/GSSG were examined in nine organs of mice of both sexes after exposed to sulphur dioxide (SO 2) at several concentrations. The results showed that: (1) at 22 mg/m 3 of SO 2 inhalation concentration, there were no significant difference between the experimental and control mice for the GSH levels and GSH/GSSG ratio in all of tested organs; (2) at 64mg/m 3, the GSH levels were significantly decreased in lungs, kidney, brain, stomach and testicle of the male mice in the experimental group, while the GSH levels and GSH/GSSG ratios in the tested organs of the female mice had no marked changes in the comparison with those in the control group; (3) 148 mg/m 3 of SO 2 inhalation led to decrease of both the GSH levels and GSH/GSSG ratios, in that the GSH levels were significantly lowered in liver, lung, kidney, heart, brain, stomach, small intestine and testicle of the male mice and in liver, lung, kidney, small intestine of the female mice, and the GSH/GSSG ratios were in liver, kidney, small intestine and testicle of the male mice and in liver, lung, kidney, brain, stomach, small intestine of the female mice. It indicated that SO 2 above certain concentration could make effects not only on respiratory organ, but also on other organs, and the susceptibility of the male and the female mice to SO 2 was different. The decline of the GSH level and GSH/GSSH ratio suggests that the toxic effect of SO 2 is associated with its lowering the levels of antioxidants and weakening the defense system and antioxidation in organisms.