维生素E同系化合物诱导人肝癌细胞凋亡的作用

Effect of apoptosis induced by different vitamin E homologous analogues in human hepatoma cells( HepG2)

为探讨α 生育酚 (α T)、γ 生育酚 (γ T)、δ 生育酚 (δ T)及维生素E琥珀酸酯 (VES) 4种维生素E同系化合物对肝癌细胞凋亡影响 ,在体外培养的人肝癌细胞 (HepG2 )培养液中分别加入 12 5、2 5 0、5 0 0、10 0 0及 2 0 0mg L的 4种维生素E同系化合物培养 48h后 ,采用流式细胞仪技术 (FCM)和DNA梯度电泳 (DNALad der)定量分析HepG2细胞凋亡及DNA降解片段情况。结果显示 ,FCM凋亡检测发现δ T和VES培养的HepG2细胞凋亡率增加 ,并呈现剂量依赖关系 ,即随着δ T、VES剂量增加HepG2细胞凋亡亦增加 ,其中δ T诱导HepG2细胞凋亡作用强于VES ;δ T处理的HepG2细胞只在 2 0 0mg L剂量时出现轻度的凋亡增加 ;未见α T有诱导HepG2细胞凋亡的作用。DNA梯度电泳检测发现δ T和VES处理HepG2细胞的DNA出现明显的排列成梯状的分子条带 ,α T、γ T处理组则未见明显的条带。结果提示 ,维生素E同系化合物诱导肝癌细胞凋亡作用不同 ,效应排序为δ T >VES >γ T >α T ,δ

To observe the effect of α tocopherol(γ T), δ tocopherol(δ T), δ tocopherol(δ T), and vitamin E succinate (VES) on apoptosis induced in human hepatoma cells (HepG2), the cell apoptosis was detected by flow cytometry (FCM) and DNA ladder under the treatment of different VE homologues analogues (α T, γ T, δ T and VES) at different concentrations (12 5, 25, 50, 100 and 200mg/L) for 48 hours. The results showed that the growth of HepG2 cells was inhibited byδ T and VES at 12 5 200 mg/L in comparison with the negative control group, while γ T showed weak effect of inhibition and α T did not show any inhibition effect. With the exception of α tocopherol, δ tocopherol and VES were effective in induction of apoptosis in HepG2 cells at concentrations of 12 5 200mg/L. γ tocopherol showed effect only at 200mg/L. δ tocopherol and VES decreased HepG2 cells growth and viability, and increased apoptotic propensity significantly. A dose dependent of antiproliferation and induction of apoptosis were found in HepG2 cells line. The order of efficiency of four vitamin E analogues was δ tocopherol >VES>γ tocopherol>α tocopherol. It is suggested that δ tocopherol and VES may be a agent that has a anti hepatomatic potential.