红曲中洛伐他汀总量的测定方法

Determination of total lovastatin in red rice

考察降脂红曲产品的安全性和功效性 ,测定洛伐他汀的总量比仅仅测定其内酯含量更为可取。本文建立了测定红曲中洛伐他汀总量的方法 ,并对方法的可行性进行了验证。采用HPLC方法 ,色谱柱 :C18柱(2 5 0mm× 4 60mm) ;流动相 :甲醇 :水 (72 :2 8) ,检测波长 :2 3 8nm ;流速 :1 0ml min;柱温 :室温 ,方法回收率98 3 % ,RSD =0 3 3 %。

In order to text effectiveness and safety of red rice products, the determination of total lovastatin is more optional than that of determination of its lactone only. The method for determination of total lovastatin is established in this study. HPLC method is applied with the C18 colume (250mm×4.60mm). Flow phase: water (72:28); wave length: 238nm; Flow speed: 1.0ml/min; colume temperature: room temerature. Moreover, the reliability of this method validated recovery rate 98 3, RSD =0 33%.