喉癌和鼻咽癌体外药敏试验AgNOR法的实验及临床研究

Experimental and clinical study of in vitro chemosensitivity assay for laryngeal carcinoma and nasopharyngeal carcinoma by AgNOR methods

目的 :用嗜银蛋白检测技术 (AgNOR)分析喉癌和鼻咽癌对 8种常用化疗药物的敏感性。 方法 :从 5 6例喉癌和鼻咽癌患者获取新鲜肿瘤组织标本 ,采用单纯机械分离法制备单细胞悬液 ,加入抗癌药物后培养 36h ,细胞悬液涂片、银染 ,计数银染颗粒后计算均值下降率 ;12例患者同期行化疗。结果 :5 1例肿瘤获得实验成功 ,可评价率为 91.1% ;AgNOR颗粒均值下降率从 0～ 6 3% ,在 8种单一药物中 ,对DDP的敏感率最高 ,其它较为敏感的药物为VCR、5 -FU、PYM ;鼻咽癌对抗癌药物的敏感性显著高于喉癌对抗癌药物的敏感性 ;体外药敏与体内疗效的总符合率为 80 %。结论 :AgNOR技术简便易行 ,与临床相关性好 ,是一种有应用前景的体外药敏筛选方法。

Objective: To evaluate in vitro chemosensitivity of laryngeal carcinoma and nasopharyngeal carcinoma to eight kinds of antineoplastic drugs by AgNOR technique. Methods: Fresh specimens of 56 cases were obtained from laryngeal carcinoma and nasopharyngeal carcinoma.We severed tumor tissue with simple mechanical separation method.After the acting of drugs,cell smear and argyrophilic staining,the mean inhibiting rate was calculated.10 cases were treated with chemotherapy at the same time. Results: 51 cases′specimens succeeded in the experiments,and the rate of success was 91.1%.The changes of mean inhibiting rate was from 0 to 63%.The chemosensitivity to DDP was the highest in eight kinds of antineoplastic drugs.Secondly,VCR,5-FU,and PYM were the highest.The sensitivity to drugs in laryngeal carcinoma was significantly higher than nasopharyngeal carcinoma.The coincidence between the experiment and clinic was 80%. Conclusion: AgNOR method is simple and convenient.In addition,it has clear correlation with the results of therapy.The method is useful for chemotherapy.