转Bt基因抗虫棉源器官的建成及其功能

The Morphological Characters and Physiological Function of Source Organs in Bt-transgenic Cotton

转Bt基因抗虫棉中棉所30的单株叶面积、叶面积系数和侧根数量较轮回亲本中棉所16大幅度降低,果枝叶输出的蔗糖、可溶性糖和氨基酸及输入棉铃的蔗糖、可溶性糖和全N较中棉所16大幅度下降。自初花期至结铃早期,中棉所30单位植株的伤流量、伤流液中的Pi、NO3-和氨基酸流量均显著或极显著低于中棉所16,反映其单株根系活力、根系吸收、合成和运输能力全面下降,但在盛花期之前,中棉所30的根系功能与地上部发育的协调性并不差(以单位地上部干重的伤流量及其组分来衡量),只是棉株开始大量结铃后,中棉所30根系与地上部的协调性迅速劣化,容易导致早衰。文中还讨论了Bt棉适宜的栽培措施,并对采用EDTA促泌法收集棉花韧皮部汁液的方法进行了评价。

The leaf area per plant, LAI and the number of lateral roots of Bt transgenic cotton (cv. CCRI 30) are much less than those of its recurrent parents (cv. CCRI 16), which make the exporting rate of sucrose, soluble sugar, amino acids and total nitrogen from sympodial leaves and the importing rate of these substances into ball decrease dramatically. The exudation rate of bleeding sap and Pi、NO3 and amino acids exudation rate in it in terms of per plant of CCRI 30 are lower significantly than those of CCRI 16 from earlyblooming stage to mass ballforming stage, which indicate the decrease of root activity, uptake ability, synthetic ability and transporting ability of CCRI 30 in terms of per plant. It is interesting that the coordination of roots and shoots in CCRI 30 is excellent before fullblooming stage ( reflected by the exudation rate of bleeding sap and Pi、NO3 and amino acids exudation rate in it in terms of dry weight of shoots), and this coordination does not become bad until ballforming stage. The proper manage measures for Bt cotton and the methods of collecting phloem sap using EDTA are also discussed. 