中国黄连组织和细胞培养条件的研究 Ⅲ、接种量、材料日龄对培养物生长和小檗碱积累的影响

STUDIES ON TISSUE AND CELL CULTURE CONDITION OF COPTIS CHINENSIS Ⅲ、 Effects of Inoculum Quantity and Material Age on Culture Growth and Berberine Accumulation

以中国黄连细胞无性系为材料,在6,7—V固体和液体培养基的基础上进行不同激素配比试验,结果为:(1)在固体培养基含KT0.1mg/L的条件下,添加2,4—D1.0～4.0mg/L能使培养物生长旺盛,2,4—D浓度再增大,生长减慢,白化透明细胞增多,小檗碱合成受到抑制;液体悬浮培养的2,4—D浓度大于1.0mg/L时,培养物干重及小檗碱含量随着2,4—D浓度的增大而下降。固体培养添加NAA1.0～10.0mg/L能提高培养物干物质积累和小檗碱含量,液体悬浮培养的NAA浓度大于1.0mg/L时,细胞培养物的小檗碱含量随着NAA浓度的增大而下降。(2)在培养基含2,4—D1.6mg/L的条件下,固体培养以添加KT或BA0.1mg/L的培养物干重和小檗碱含量为高,液体悬浮培养物的小檗碱含量则随KT或BA浓度(0.01～1.00mg/L)的增大而提高。在固体培养方式下对培养物生长和小檗碱积累较为有利的激素配比为KT0.1mg/L+NAA2.0～4.0mg/L;在液体悬浮培养方式下对培养物生长和小檗碱积累较为有利的激素配比为KT0.01mg/+NAA1.0mg/L。

The effects of various phytohormone combination supplemented to 6,7-V basic medium on culture growth and berberin accumulation of Coptis Chinensis were studied. A suitable prescriptionoting for prom oting both dry matter yield andberberin accumulatian was found. That is KT 0.1mg/L + NAA 2-4mg/L under solid culture condition or KT 0.01mg/L + NAA 1.0mg/L under liguid shake culture condition.