海藻酸钠/壳聚糖微胶囊包埋人卵巢癌细胞腹腔移植的实验研究

Experimental study of the abdominal cavity transplantation of alginate-chitosan microencapsulated SKOV3 cells

目的 探讨用改性壳聚糖代替聚赖氨酸 ,制备海藻酸钠 /壳聚糖 (ACA)微胶囊用于细胞移植的可行性。方法 使用大功率高压脉冲微胶囊制备仪 ,制备包埋人卵巢癌 (SKOV3)细胞的ACA微胶囊 ,然后采用注射的方法将其移植到小鼠的腹腔。结果 包埋SKOV3细胞的ACA微胶囊移植后 ,在小鼠的腹腔内保持了原有的形状和结构 ,囊内的细胞正常生存并保持增殖功能。结论 壳聚糖作为制备微胶囊的材料 ,具有良好的生物相容性和机械强度。因此 ,可望代替价格昂贵的聚赖氨酸制备微胶囊 ,作为免疫隔离工具在组织细胞移植技术的发展中发挥重要作用。

Objective To investigate the feasibility of the replacement of poly-l-lysine with chitosan to prepare microcapsules used for cell transplantation.Methods The alginate-chitosan microcapsules (ACA) entrapping SKOV3 cells with droplet generator were prepared and injected into the abdominal cavity of mice. Results ACA microcapsules could remain intact after transplantation for a long time. The entrapped SKOV3 cells survived and the proliferation function of cells were maintained.Conclusions Chitosan is a polysaccharide material with good biocompatibility. The in vivo experiment results show that ACA microcapsules have the enough mechanic strength. It is possible for chitosan to replace poly-l-lysine in microcapsule preparation and to exert important function in cell transplantation technology.