幽门螺杆菌感染对学龄前儿童铁营养状况的影响

Effect of Helicobacter pylori infection on iron status among preschool children

目的 探讨Hp感染对儿童铁营养状况的影响。方法 对 475名 2～ 7岁儿童进行膳食和社会经济状况调查、外周血血细胞分析、血清铁蛋白及Hp抗体检测。对血清Hp抗体阳性者进行粪便Hp抗原测定和粪便潜血试验。结果 64名儿童感染Hp ,3 0 5名儿童未感染。感染Hp儿童血清铁蛋白水平明显低于未感染Hp儿童。两组调整后的血清铁蛋白均值及 95 %可信区间分别为 2 3 62μg/L (7 13 μg/L～ 78 2 6μg/L)、3 3 48μg/L(10 2 8μg/L～ 10 9 0 6μg/L)。以非条件logistic多元回归模型有效平衡其他因素的混杂偏倚后 ,Hp感染仍是儿童铁缺乏的危险因素 ,其OR值为 7 95 (OR 95 %CI为 2 5 6～ 2 4 67)。结论 Hp感染儿童机体铁营养水平降低 。

Objective To explore the relationship between Helicobacter pylori (Hp) infection and iron status using serum ferritin(SF) as a marker for total iron and to identify the related factors of iron nutritional status among preschool children Methods By cluster sampling, we recruited 475 preschool children aged 2 to 7 years A structured questionnaire and diet form were sent to the parents of these children to obtain related information about the socioeconomic level and dietary intakes After collecting blood samples, the following indexes were measured Hp IgG antibodies were measured with a dot enzyme linked immunoassay; hemoglobin, Hct, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width index (RDW) with automatic Complete Blood Count; SF with an immunoradiometric assay Stool Hp antigen and occult bleeding were measured with ELISA among individuals who were Hp seropositive Hp status was defined as positive when both serum and stool antigen tests were positive, Hp status was defined as negative when serum antigen test was negative; 24 hour weighting and recording methods were used to dietary survey for three days in May and December 2001, respectively, dietary intakes including energy, protein and micronutrient were calculated using nursery school nutrition software and evaluated by Chinese Dietary Reference Intakes (DRIs) Mann Whitney test was used to compare mean ranks of SF in Hp positive and Hp negative children To obtain an adjusted estimate of the impact of Hp infection on SF, a multivariate analysis of covariance was done to evaluate the different level of SF between Hp infected and non infected status The relationship between iron deficiency and gender, age, socioeconomic condition, iron intake, and calcium intake was assessed by univariate analysis An unconditional multivariate logistic regression analysis was also performed Iron deficiency status was dichotomized and placed as the dependent variable Hp infection status was incorporated together with possible confounding factors as independent variables in a final logistic regression model All the data were managed by EPI Info 5 01a and analyzed by SAS (Version 6 12) Results Totally 64 children were diagnosed as Hp positive and 305 as Hp negative Mann Whitney test and multivariate analysis of covariance both showed that SF concentration was significantly lower in Hp infected individuals than non infected individuals Adjusted mean level and 95% confidence interval of SF in infected and non infected children was 23 62 μg/L (7 13 μg/L-78 26 μg/L), 33 48 μg/L (10 28 μg/L-109 06 μg/L), respectively The relationship between Hp infection and iron deficiency status persisted in logistic regression analysis after adjusting for possible confounding factors (OR:7 95; 95%CI 2 56-24 67) Conclusion Iron nutritional status was reduced in Hp infected preschool children Hp infection appears to be an independent risk factor or an added stressor on iron status among preschool children