肌营养不良蛋白聚糖复合物在RT4细胞中的表达

Expression of dystroglycan complex in RT4 cells

目的 观察大鼠神经鞘瘤细胞 (RT4)中肌营养不良蛋白聚糖复合物的表达状况。方法 常规培养RT4细胞 ,应用免疫荧光染色、蛋白电泳、免疫印迹等方法观察肌营养不良蛋白聚糖复合体在 RT4细胞中的表达并鉴定分子量。结果 肌营养不良蛋白聚糖复合体表达于 RT4细胞的细胞膜上。结论 肌营养不良蛋白聚糖复合体对于

Objective To examine the expression of dystroglycan complex in the membrane of rat's schwannoma cell (RT4). Methods By the means of immunofluorescence,SDS PAGE and immunoblot assays the expression of dystrolgycan complex in RT4 cells. Results The dystroglycan complex was observed expressing on RT4 cell membrane and molecular weight was tested. Conclusion The dystroglycan complex provides a tight link between the ECM and the membrane of RT4 cell.