摘要
目的 探讨脑挫伤早期细胞凋亡相关基因Bcl—2、Fas/Fas—L的表达变化规律及神经细胞凋亡的调控机制。方法 运用光镜观察脑挫伤后脑组织的形态学变化,同时应用免疫组织化学方法检测脑挫伤早期不同时程Bcl-2、Fas/Fas-L的表达变化规律。结果 伤后8h在脑挫伤周围可见典型的凋亡神经细胞;伤后30min损伤灶周围神经细胞出现Bcl-2、Fas/Fas-L阳性表达,以后呈逐渐上升趋势且表达范围亦逐渐扩大,其中Bcl-2蛋白表达在伤后4h达高峰,随后呈下降的趋势,至伤后10～12h其表达程度与伤后1h相比无明显差异。结论 神经细胞凋亡参与脑挫伤后病理形态学改变,神经细胞调亡的形成可能与促凋亡调控基因和抑调亡调控基因的表达不平衡有关。
Objective To investigate the expressions of apoptotic regulating gene Bcl-2 and Fas/Fas - L and the molecular mechanism of neuronal apoptosis in the earlier period of cerebral contusion. Methods The morphologia feature of brain tissue was observed under microscopy and immunohistochemistry technique was applied to detect the expression of Bcl-2 and Fas/ Fas- L. Results The typical apoptotic regulating gene was noted in 30 minutes and kept increasing subsequently. The expression of Bcl-2 reached maximum level in 4 hours and then declined. Conclusion Cerebral contusion can facilitate neuronal apoptosis. The mechanism of neuronal apoptosis is correlative with the unbalanced expressions of apoptotic regulating genes.
出处
《中国煤炭工业医学杂志》
2003年第4期376-378,共3页
Chinese Journal of Coal Industry Medicine
关键词
脑挫伤
细胞凋亡
基因表达
cerebral contusion
Bcl-2
Fas/Fas- L
apoptosis
